1 Oral and Maxillofacial Radiology Specialist Study Program, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.
2 Department of Dentomaxillofacial Radiology, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta, Indonesia.
World Journal of Advanced Research and Reviews, 2025, 27(01), 090-094
Article DOI: 10.30574/wjarr.2025.27.1.2497
Received on 07 April 2025; revised on 28 June 2025; accepted on 30 June 2025
Objective: Previous studies have indicated that the number of micronucleus, a marker of DNA damage, is increased in gingival mucosa after a panoramic examination. β-Carotene is the lipid-soluble antioxidant substance and expected to prevent the oxidative reaction by improves the junctional communication and reduce the risk from DNA damage. The objective of this study was to monitor the changes of micronucleus during panoramic examination by applying β-Carotene Patch to rabbit gingiva.
Methods: A total of 18 New Zealand rabbits were divided into 2 groups equally. Group, I was the control group that was given a panoramic examination without applying β-Carotene Patch. Group II was the test group that applied β-Carotene Patch before the panoramic examination. Micronucleus samples were taken from rabbit’s gingival mucosa then stained by the method of modified Feulgen-Rosenbeck. The results were analyzed using one-way ANOVA (P < 0.05).
Results: The number of micronucleus in Group I was significantly higher than those in the Group II (P = 0.001).
Conclusion: β-Carotene Patch may reduce the number of micronucleus formation of rabbit’s gingival epithelial cells exposed to panoramic radiography.
β -Carotene; Patch; Micronucleus; Panoramic Examination; Gingival Mucosa
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Rurie Ratna Shantiningsih, Munakhir Mudjosemedi and Eha Renwi Astuti. Effect of β-Carotene patch application on micronucleus formation of gingival epithelial cells exposed to panoramic radiography, an in vivo study. World Journal of Advanced Research and Reviews, 2025, 27(01), 090-094. Article DOI: https://doi.org/10.30574/wjarr.2025.27.1.2497.
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