1 Physical Sciences Program, School of Arts, Sciences, and Education, Ivy Tech Community College, South Bend, Indiana, USA.
2 School of Biomedical Sciences, Faculty of Health, Queensland University of Technology, Australia.
3 Department of Applied Medical Chemistry, Medical Research Institute, Alexandria University, Alexandria, Egypt
3 Department of Medical Laboratory Analysis, Faculty of Medical & Health Sciences, Liwa College, Abu Dhabi, UAE
4 Noon’s Clinic, Muscat, Oman.
5 Royal Hospital, Oman (Former), Kilkenny, Ireland.
World Journal of Advanced Research and Reviews, 2025, 25(03), 406-410
Article DOI: 10.30574/wjarr.2025.25.3.0725
Received on 26 January 2025; revised on 03 March 2025; accepted on 05 March 2025
Aim: This study aims to evaluate the antioxidant capacity of black tea, turmeric, melatonin, and chamomile/lavender tea using the iodine clock reaction, a kinetic method for assessing antioxidant activity.
Background: Oxidative stress, caused by an imbalance between free radicals and antioxidants, contributes to chronic diseases, including cardiovascular diseases and neurodegenerative disorders. Antioxidants play a crucial role in mitigating oxidative damage.
Methods: The iodine clock reaction was utilized to assess antioxidant activity by measuring the delay in the oxidation process. Reaction times were recorded for each antioxidant sample, and statistical analysis (P-test) was performed to determine significance compared to the control.
Results: Black tea exhibited the longest reaction delay (612.47s), indicating the highest antioxidant potential, followed by melatonin (344.75s), chamomile/lavender tea (248.98s), and turmeric (200.56s). Statistical analysis confirmed significant differences in antioxidant efficiencies (P < 0.05).
Conclusion: The results suggest that black tea is the most potent antioxidant among the tested samples, supporting its health benefits. The iodine clock reaction proves to be a reliable method for antioxidant evaluation. Further research should explore additional antioxidants and optimize experimental conditions.
Antioxidant activity; Iodine clock reaction; Oxidative stress; Chemical kinetics; Dietary antioxidants; P-test analysis; Free radicals
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Hassan Darwish, Danielle Parker, Evan Brewer, Harajit Rao, Julisa Laris, Hiba Al Lawati, Hagar M. Mohamed, Manohar Noone and Una Gibbons. Comparative antioxidant analysis using the iodine clock reaction: A kinetic approach. World Journal of Advanced Research and Reviews, 2025, 25(03), 406-410. Article DOI: https://doi.org/10.30574/wjarr.2025.25.3.0725.
Copyright © 2025 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0