Ameliorative potentials of Kolaviron and Brophyllum pinnatum extract on IBA-1, GFAP, cytochrome p450 and xanthine oxidase in AlCl3-induced neurotoxicity in rats

This study aimed at investigating the comparative effect of kolaviron and ethanolic leaf extract of Brophyllum pinnatum on Aluminium Chloride induced Neurotoxicity in male Wistar rats. Thirty-five (35) Wistar rats weighing 150-250g were randomly divided into 7 groups of 5 rats each. Group 1 as the control group, group 2 (Aluminium chloride) was given AlCl₃ orally at a dose of 100mg/kg btw. Group 3 (kolaviron (kv) only) was given 200mg/kg bwt of Kv. Group 4 (Brophyllum pinnatum only) was given 600mg/kg bwt of ethanolic extract of B. pinetum (Bp). Group 5 (AlCl₃ + Bp). Group 6 (AlCl₃ + Kv), and Group 7 (AlCl₃ + Bp + Kv). All groups took normal rat chow and water ad libitum for 28 days. The results showed a significant (p<0.05) increase in the serum level of CYP450 in AlCl₃ (1.30±0.12) when compared with the control (0.15±0.03), AlCl₃+Bp (0.55±0.03), AlCl₃+Kv (1.00±0.23) and AlCl₃+Kv+Bp (1.00±0.01). The serum level of xanthine oxidase in AlCl₃ was 1.65±0.09, showing a significant (p<0.05) increase in Xanthine oxidase compared with the control (0.15±0.03), and other experimental groups. A significant (p<0.05) increase in GFAP levels was recorded in AlCl₃ (24.8±2.5) compared with control and other groups. Also, significant increase in IBA-1 level was recorded in the AlCl₃ (24.8±2.5) compared with control and other experimental groups. In conclusion, administration of Brophyllum pinnatum and kolaviron ameliorated the neurotoxicity induced by AlCl₃, probably via their anti-oxidative, anti-inflammatory and anti-apoptotic potentials.